Can Immobilized Artificial Membrane Chromatography Support the Characterization of Antimicrobial Peptide Origin Derivatives?

Antibiotics

Wojciech Kamysz¹  Krzysztof Rolka²  Anna Łęgowska²  Natalia Ptaszyńska²  Hanna Kapica³  Katarzyna E. Greber³  Monika Pastewska³  Krzesimir Ciura³  Wiesław Sawicki³ 

[1] Department of Inorganic Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland;Department of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Al. Gen. Hallera 107, 80-308 Gdańsk, Poland;Department of Physical Chemistry, Medical University of Gdańsk, Al. Gen. Hallera 107, 80-416 Gdańsk, Poland;

关键词: IAM-HPLC;    antimicrobial peptide;    cell-penetrating peptide (CPP);    transportan 10 (TP10-NH2);    short cationic lipopeptides;   

DOI  :  10.3390/antibiotics10101237

来源: DOAJ

【 摘 要 】

The emergence and spread of multiple drug-resistant bacteria strains caused the development of new antibiotics to be one of the most important challenges of medicinal chemistry. Despite many efforts, the commercial availability of peptide-based antimicrobials is still limited. The presented study aims to explain that immobilized artificial membrane chromatography can support the characterization of antimicrobial peptides. Consequently, the chromatographic experiments of three groups of related peptide substances: (i) short cationic lipopeptides, (ii) citropin analogs, and (iii) conjugates of ciprofloxacin and levofloxacin, with a cell-penetrating peptide were discussed. In light of the discussion of the mechanisms of action of these compounds, the obtained results were interpreted.

【 授权许可】

Unknown