Efficient Improving the Activity and Enantioselectivity of Candida rugosa Lipase for the Resolution of Naproxen by Enzyme Immobilization on MCM-41 Mesoporous Molecular Sieve

International Journal Bioautomation

Yi Xu¹  Xiao-mei Wu¹  Ying Chen² 

[1] ;School of Chemical and Environmental Engineering, Shanghai Institute of Technology, 100, Hai Quan Road, Shanghai, China, 201418;

关键词: MCM-41;    Immobilization;    Lipase;    Enantioselectivity;    Naproxen;    Stability;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lipase from Candida rogusa was immobilized on MCM-41 mesoporous molecular sieves in a trapped aqueous-organic biphase system for the resolution of racemic naproxen methyl ester. It was interesting that the activity and enantioselectivity of the immobilized lipase were improved significantly relative to the free enzyme. The proportion of water (ml)/support (g) has a dramatic influence on the activity and enantioselectivity of lipase immobilized onto MCM-41 molecular sieves. It was also found that the activity of immobilized lipase was more sensitive to pH value and temperature than the free one. Higher pH value will increase the activity but decrease the enantioselectivity of the immobilized lipase. The enantioselectivity of the immobilized lipase was not altered significantly within the range of tested temperature. The immobilized lipase can be reused for at least 8 batches without significant lose of activity with the aid of methanotrophic bacteria to eliminate the methanol produced during the resolution process.

【 授权许可】

Unknown