Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Plant Methods

Meirav Leibman-Markus¹  Maya Bar¹  Rupali Gupta¹  Ziva Amsalem²  Lior Tal²  Alon Israeli³  Evyatar Steiner³  Mika Farber³  Naomi Ori³  Ido Shwartz³  Ido Nir³  Bruno Müller⁴ 

[1] Department of Plant Pathology and Weed Research, Plant Protection Institute, Agricultural Research Organization;Department of Plant and Environmental Science, Weizmann Institute of Science;Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem;Leibniz-Institut Für Pflanzengenetik Und Kulturpflanzenforschung (IPK);

关键词: Cytokinin;    Imaging;    Two-component sensor (TCS);    TCSv2;    Tomato;    Arabidopsis;   

DOI  :  10.1186/s13007-020-00694-2

来源: DOAJ

【 摘 要 】

Abstract Background Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

【 授权许可】

Unknown