Downregulation of miRNA‐126‐3p is associated with progression of and poor prognosis for lung squamous cell carcinoma

FEBS Open Bio

Shu‐Ping Huang¹  Ting‐Qing Gan¹  Xiao‐Jiao Li²  Wan‐Ying Huang³  Wei‐Jia Mo³  Xiang‐Ming Wang³  Peng Chen³  Li Gao³  Zu‐Yun Li³  Gang Chen³  Zhi‐Guang Huang³  Hui‐Ping Lu³  Gao‐Qiang Zhai³  Da‐Ping Yang⁴  Jie Yang⁵  Hua‐Fu Zhou⁶  Jiao Luo⁶  Shang‐Wei Chen⁶  Jun Liu⁶  Zong‐Wang Fu⁶ 

[1] Department of Medical Oncology The Second Affiliated Hospital of Guangxi Medical University Nanning China;Department of PET/CT First Affiliated Hospital of Guangxi Medical University Nanning China;Department of Pathology First Affiliated Hospital of Guangxi Medical University Nanning China;Department of Pathology Guigang People's Hospital of Guangxi/the Eighth Affiliated Hospital of Guangxi Medical University Guigang China;Department of Pharmacology School of Pharmacy Guangxi Medical University Nanning China;Department of Thoracic and Cardiovascular Diseases First Affiliated Hospital of Guangxi Medical University Nanning China;

关键词: lung squamous cell carcinoma;    MiRNA‐126‐3p;    RT‐qPCR;    target genes;    TCGA;   

DOI  :  10.1002/2211-5463.12920

来源: DOAJ

【 摘 要 】

Lung squamous cell carcinoma (LUSC) is the main pathological type of pulmonary malignant tumors; at present, less than 10% of patients with advanced metastatic LUSC live for more than 5 years. We previously reported that low expression of miRNA‐126‐3p is associated with the occurrence and progression of lung adenocarcinoma (LUAD). Here, we examined expression of miRNA‐126‐3p in 23 samples from patients with LUSCs and 23 normal control specimens by quantitative real‐time PCR (RT‐qPCR). Associations between miRNA‐126‐3p expression and clinical features were studied from materials derived from Gene Expression Omnibus (GEO) chips and The Cancer Genome Atlas (TCGA) database. Twelve online platforms were used to identify candidate target genes of miRNA‐126‐3p. Further analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and protein–protein interaction (PPI) network were performed on the target genes. GEO microarray analysis, TCGA data mining, RT‐qPCR, and integration analysis consistently reported low expression of miRNA‐126‐3p in LUSC. A total of 42 genes were identified as potential target genes of miRNA‐126‐3p from online platforms, GEO microarrays, and the TCGA database. GO and KEGG analyses demonstrated that the target genes are involved in several biological processes that promote the progression of LUSC. SOX2, E2F2, and E2F3 were selected as hub genes from the PPI network for further analysis. In summary, our results suggest that the low expression of miRNA‐126‐3p may play a role in promoting the development of LUSC and miRNA‐126‐3p may be a biomarker for LUSC early diagnosis and prognosis.

【 授权许可】

Unknown