Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules

Biosensors

Christophe A. Marquette¹  Loïc J. Blum¹  Ophélie I. Berthuy¹ 

[1] Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires—Institut de Chimie et Biochimie Moléculaires et Supramoléculaires—Université Claude Bernard Lyon 1, Villeurbanne 69100, France;

关键词: biosensor;    cell microarray;    cell encapsulation;    SPRi;    real-time;   

DOI  :  10.3390/bios6010002

来源: DOAJ

【 摘 要 】

In the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip.

【 授权许可】

Unknown