Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm

Biomolecules

Celia Llamazares¹  Juan Soliveri¹  JoséLuis Copa-Patiño¹  Natalia Sanz del Olmo²  Paula Ortega²  F.Javier de la Mata²  Rafael Gómez²  Sandra García-Gallego² 

[1] Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Madrid, Spain;Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry “Andrés M. del Río” (IQAR), University of Alcalá, 28805 Madrid, Spain;

关键词: dendrimer;    metallodendrimer;    metal;    copper;    ruthenium;    antibacterial;    biofilm;    Staphylococcus aureus;    Escherichia coli;   

DOI  :  10.3390/biom9090405

来源: DOAJ

【 摘 要 】

Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II) and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of S. aureus biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of S. aureus biofilms, but none produce hemolysis at the inhibitory concentrations and can be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms.

【 授权许可】

Unknown