Insights into Emerging Begomovirus–Deltasatellite Complex Diversity: The First Deltasatellite Infecting Legumes

Biology

Liseth Bastidas¹  Dorys T. Chirinos²  Elvira Fiallo-Olivé³  Jesús Navas-Castillo³ 

[1] Departamento Fitosanitario, Facultad de Agronomía, Universidad del Zulia, Maracaibo 4005, Zulia, Venezuela;Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí, Portoviejo 130105, Manabí, Ecuador;Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Consejo Superior de Investigaciones Científicas, Avenida Dr. Wienberg s/n, 29750 Algarrobo-Costa, Málaga, Spain;

关键词: Geminiviridae;    begomoviruses;    deltasatellites;    legumes;    black gram;    common bean;   

DOI  :  10.3390/biology10111125

来源: DOAJ

【 摘 要 】

Begomoviruses and associated DNA satellites are involved in pathosystems that include many cultivated and wild dicot plants and the whitefly vector Bemisia tabaci. A survey of leguminous plants, both crops and wild species, was conducted in Venezuela, an understudied country, to determine the presence of begomoviruses. Molecular analysis identified the presence of bipartite begomoviruses in 37% of the collected plants. Four of the six begomoviruses identified constituted novel species, and two others had not been previously reported in Venezuela. In addition, a novel deltasatellite (cabbage leaf curl deltasatellite, CabLCD) was found to be associated with cabbage leaf curl virus (CabLCV) in several plant species. CabLCD was the first deltasatellite found to infect legumes and the first found in the New World to infect a crop plant. Agroinoculation experiments using Nicotiana benthamiana plants and infectious viral clones confirmed that CabLCV acts as a helper virus for CabLCD. The begomovirus–deltasatellite complex described here is also present in wild legume plants, suggesting the possible role of these plants in the emergence and establishment of begomoviral diseases in the main legume crops in the region. Pathological knowledge of these begomovirus–deltasatellite complexes is fundamental to develop control methods to protect leguminous crops from the diseases they cause.

【 授权许可】

Unknown