Efficient Phytase Secretion and Phytate Degradation by Recombinant Bifidobacterium longum JCM 1217

Frontiers in Microbiology

Maoping Song¹  Xinqi Hao¹  Zhongke Sun¹  Xingdong Yang²  Cuiwei Chu²  Zonghao Yue²  Can Chen²  Chengwei Li³  Lili Li³ 

[1] College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China;College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou, China;Key Laboratory of Plant Molecular Breeding and Bioreactor, Zhoukou, China;

关键词: Bifidobacterium longum JCM 1217;    promoter;    phytase;    phytate;    phosphorus;   

DOI  :  10.3389/fmicb.2019.00796

来源: DOAJ

【 摘 要 】

Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous appA and is stable within Bifidobacterium longum JCM 1217. Cloning of three predicted promoters into pBPES proved that all of them drive appA expression in B. longum JCM 1217. Transformation of plasmids pBPES-tu and pBPES-groEL into B. longum JCM1217 resulted in much more phytase secretion suggests Ptu and PgroEL are strong promoters. Further in vitro and in vivo experiments suggested B. longum JCM 1217/pBPES-tu degrades phytate efficiently. In conclusion, the study screened two stronger promoters and constructed a recombinant live probiotic strain for effectively phytase secretion and phytate degradation in gut. The strategy used in the study provided a novel technique for improving the bioaccessibility of phytate and decreasing phosphorus excretion.

【 授权许可】

Unknown