Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans

Metabolites

Christian-Alexander Dudek¹  Lisa Schlicker²  Gang Zhao²  Arnab Barua³  DorisM. Jacobs³  Jean-Pierre Trezzi³  Michael Meyer-Hermann⁴  HannyM. Boers⁵  Karsten Hiller⁵ 

[1] D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands;Department for Bioinformatics and Biochemistry, BRICS, Technische Universität Braunschweig, Rebenring 56, 38106 Braunschweig, Germany;Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Rebenring 56, 38106 Braunschweig, Germany;Integrated Biobank of Luxembourg, Luxembourg Institute of Health, 1 rue Louis Rech, 3555 Dudelange, Luxembourg;;Unilever R&

关键词: ¹³C-enrichment;    GC-MS;    wheat flour;    guar gum;    chickpea flour;    metabolism;    starch;   

DOI  :  10.3390/metabo9050091

来源: DOAJ

【 摘 要 】

Food supplementation with a fiber mix of guar gum and chickpea flour represents a promising approach to reduce the risk of type 2 diabetes mellitus (T2DM) by attenuating postprandial glycemia. To investigate the effects on postprandial metabolic fluxes of glucose-derived metabolites in response to this fiber mix, a randomized, cross-over study was designed. Twelve healthy, male subjects consumed three different flatbreads either supplemented with 2% guar gum or 4% guar gum and 15% chickpea flour or without supplementation (control). The flatbreads were enriched with ~2% of ¹³C-labeled wheat flour. Blood was collected at 16 intervals over a period of 360 min after bread intake and plasma samples were analyzed by GC-MS based metabolite profiling combined with stable isotope-assisted metabolomics. Although metabolite levels of the downstream metabolites of glucose, specifically lactate and alanine, were not altered in response to the fiber mix, supplementation of 4% guar gum was shown to significantly delay and reduce the exogenous formation of these metabolites. Metabolic modeling and computation of appearance rates revealed that the effects induced by the fiber mix were strongest for glucose and attenuated downstream of glucose. Further investigations to explore the potential of fiber mix supplementation to counteract the development of metabolic diseases are warranted.

【 授权许可】

Unknown