Prognostic Relevance of HJURP Expression in Patients with Surgically Resected Colorectal Cancer

International Journal of Molecular Sciences

HanJo Kim¹  Dongjun Jeong²  TaeSung Ahn³  Moo-Jun Baek³  DongHyun Kang³  HyoungJong Kwak⁴  Chang-Jin Kim⁴  Gunn Jaygal⁵  SooYoun Kim⁵  Hyeongjoo Kim⁵  Sanghee Ji⁵  Jongsoo Woo⁵ 

[1] Department of Oncology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 31151, Korea;Department of Pathology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 31151, Korea;Department of Surgery, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 31151, Korea;Research Institute of Clinical Medicine, Woori Madi Medical Center, 111 Baekjedae-ro, Wansan-gu, Jeonju, Jeollabuk-do 55082, Korea;Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University 31 Soonchunhyang 6 gil, Dongnam-gu, Cheonan, Chungcheongnam-do 330-722, Korea;

关键词: Colorectal cancer (CRC);    HJURP;    prognostic biomarker;   

DOI  :  10.3390/ijms21217928

来源: DOAJ

【 摘 要 】

HJURP is a key factor for CENP-A deposition and maintenance in centromeres. The role of mis-regulation of histone chaperones in cancer initiation and progression has been studied. However, its role in colorectal cancer is still unclear. In this study, we aimed to evaluate the expression of HJURP in 162 colorectal cancer tissue. To investigate the function of HJURP in the colorectal cancer cell, we suppressed HJURP expression by siRNA and confirmed proliferation, migration, invasion, and anchorage independent of colony forming ability. The association between HJURP expression levels and clinicopathological factors was evaluated in 162 CRC tissues using immunohistochemistry. The overall survival rate in patients of HJURP high expression was higher than those in HJURP low expression in CRC. Suppressing HJURP expression decreased cellular proliferation, invasion, and migration in four CRC cell lines: HT29, HCT116, SW480, SW620 in vitro study. Our findings revealed that the knockdown of HJURP suppressed the proliferation, migration, invasion, and tumorigenicity in CRC cells. Due to its strong association with CRC, HJURP could be a potential prognostic biomarker and a novel target for drug discovery.

【 授权许可】

Unknown