Keeping in Touch with Type-III Secretion System Effectors: Mass Spectrometry-Based Proteomics to Study Effector–Host Protein–Protein Interactions

International Journal of Molecular Sciences

MargauxDe Meyer¹  JorenDe Ryck¹  PetraVan Damme¹  Sofie Goormachtig² 

[1] Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium;VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Zwijnaarde, Belgium;

关键词: AP-MS;    BioID;    effectors;    infection biology;    interactomics;    type-III secretion;   

DOI  :  10.3390/ijms21186891

来源: DOAJ

【 摘 要 】

Manipulation of host cellular processes by translocated bacterial effectors is key to the success of bacterial pathogens and some symbionts. Therefore, a comprehensive understanding of effectors is of critical importance to understand infection biology. It has become increasingly clear that the identification of host protein targets contributes invaluable knowledge to the characterization of effector function during pathogenesis. Recent advances in mapping protein–protein interaction networks by means of mass spectrometry-based interactomics have enabled the identification of host targets at large-scale. In this review, we highlight mass spectrometry-driven proteomics strategies and recent advances to elucidate type-III secretion system effector–host protein–protein interactions. Furthermore, we highlight approaches for defining spatial and temporal effector–host interactions, and discuss possible avenues for studying natively delivered effectors in the context of infection. Overall, the knowledge gained when unravelling effector complexation with host factors will provide novel opportunities to control infectious disease outcomes.

【 授权许可】

Unknown