Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

Forests

Pei Li¹  Juncheng Li²  Xiaomei Deng²  Xin Zhan²  Wenting Qu²  Ruiqi Pian²  Xiaoyang Chen²  Junjie Zhang²  Mingqian Liu²  Boyong Liao²  Qingmin Que²  Kunxi Ouyang² 

[1] College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China;College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong 510642, China;

关键词: Toona ciliata Roem.;    SRAP markers;    PIC;    genetic diversity;   

DOI  :  10.3390/f6041094

来源: DOAJ

【 摘 要 】

Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from the species’ distribution area in China. To analyze the polymorphism in the SRAP profiles, 1505 primer pairs were screened and 24 selected. A total of 656 SRAP bands ranging from 100 to 1500 bp were acquired, of these 505 bands (77%) were polymorphic. The polymorphism information content (PIC) values ranged from 0.32 to 0.45, with an average of 0.41. An analysis of molecular variance (AMOVA) indicated that the most significant variation was attributable to differences among the populations and that variation within the populationswas small. STRUCTURE analysis divided the 30 populations into two parts. The unweighted pair group method of arithmetic averages (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the 30 populations could be classified into four types. The results demonstrate a clear geographical trend for T. ciliata in China and provide a theoretical basis for future breeding and conservation strategy of T. ciliata.

【 授权许可】

Unknown