Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression

Journal of Lipid Research

John M. Greene¹  Johan Ericsson²  Charles Florence³  Kenneth C. Carter³  Peter A. Edwards³  Brenda K. Shell³  D. Roxanne Duan³ 

[1] Medicine, University of California Los Angeles, Los Angeles, CA 90095;Departments of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095;Human Genome Sciences, Bioinformatics, 9410 Key West Avenue, Rockville, MD 20850;

关键词: prenyltransferase;   

DOI  :  

来源: DOAJ

【 摘 要 】

We report the nucleotide sequence of human geranylgeranyl diphosphate (GGPP) synthase cDNA isolated from a fetal heart library. The 2.5 kb cDNA encodes a protein of 34 kDa. The protein contains six domains that have been identified previously in many other prenyltransferases. Recombinant, purified histidine-tagged protein exhibited the enzymatic properties associated with GGPP synthase, namely the synthesis of GGPP from farnesyl diphosphate and isopentenyl diphosphate. Transient transfection of mammalian cells with a plasmid encoding the putative GGPP synthase resulted in a 55-fold increase in GGPP synthase activity. Taken together, these results establish that the cDNA encodes the mammalian GGPP synthase protein. The mRNA for GGPP synthase was expressed ubiquitiously. Of the 16 human tissues examined, the highest expression of the mRNA was in testis. The mRNA levels in cultured HeLa cells were unaffected by alterations in cellular sterol levels and contrasted with the significant regulation of isopentenyl diphosphate synthase mRNA under these same conditions. Fluorescent in situ hybridization was used to map the single gene encoding human GGPP synthase to chromosome 1q43.—Ericsson, J., J. M. Greene, K. C. Carter, B. K. Shell, D. R. Duan, C. Florence, and P. A. Edwards. Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping, and tissue expression.

【 授权许可】

Unknown