The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles

International Journal of Fertility and Sterility

Daniela Paes de Almeida Ferreira Braga¹  Edson Jr Borges¹  Ismael Dale Cotrim Guerreiro da Silva²  Amanda Souza Setti²  Assumpto Jr Iaconelli³  Tatiana Carvalho de Souza Bonetti³ 

[1] Fertility - Assisted Fertilization Center, Sao Paulo, Brazil;Molecular Gynecology Laboratory, Departament of Gynecology, University Federal of São Paulo, Sao Paulo - SP, Brazil;Sapientiae Institute, Educational and Research Center in Assisted Reproduction, Sao Paulo, Brazil;

关键词: oocyte retrieval;    ovarian stimulation;    vacuolization;    intracytoplasmic sperm injection;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background:This study compares the developmental capacity of gametes retrieved from thelargest follicle with small follicles of a cohort in controlled ovarian stimulated cycles.Materials and Methods:This prospective study performed in a private assisted fertilizationcenter included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperminjection (ICSI). After follicular aspiration, oocytes were assigned to two groups accordingto the diameter of the derived follicle. The large follicle group (n=96) comprised oocytesderived from the leading follicle of the cohort and the small follicle group (n=920) consistedoocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topqualityembryos were compared between groups by Chi-square or Fisher’s exact test, whereappropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binarylogistic regression.Results:A significantly higher percentage of oocytes derived from the leading follicle were inthe metaphase II (MII) stage (100 vs. 70.0%, p < 0.001). However we observed no significantdifferences regarding the percentage of degenerated oocytes between the large (6.25%) and smallfollicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in theincidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046).The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs.76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However,the percentage of abnormal fertilized oocytes was equally distributed between the large follicle(15.0%) and small follicle (12. 8%) groups (p=0.550).Conclusion:Our data suggest that intrafollicular mechanisms within the larger follicle of the cohortmay allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads tothe formation of more competent oocytes with higher fertilization and developmental capacities.

【 授权许可】

Unknown