A Nuclear Stress Pathway that Parallels Cytoplasmic Stress Granule Formation

iScience

Denise Campuzano¹  Antoine Gaudreau-Lapierre¹  Tyler Quoc-Thai Do¹  Claire Simada Aeschimann¹  Laura Trinkle-Mulcahy¹  Virginia Maria Ferreira Resende¹  Carmen G. Palii²  Majorie Brand² 

[1] Department of Cellular and Molecular Medicine and Ottawa Institute of Systems Biology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada;Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8L6, Canada;

关键词: Proteomics;    Optical Imaging;    Cell Biology;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Stress adaptation is exploited by cancer cells to survive and proliferate under adverse conditions. Survival pathways induced by stress are thus highly promising therapeutic targets. One key pathway involves formation of cytoplasmic stress granules, which regulate the location, stability, and translation of specific mRNAs. Here, we describe a transcriptional stress response that is triggered by similar stressors and characterized by accumulation of RepoMan (cell division cycle associated 2) at nuclear stress foci (nucSF). Formation of these structures is reversible, and they are distinct from known nuclear organelles and stress bodies. Immunofluorescence analysis revealed accumulation of heterochromatic markers, and increased association of RepoMan with the adenylate cyclase 2 (ADCY2) gene locus in stressed cells accompanied reduced levels of ADCY2 mRNA and protein. Quantitative comparison of the RepoMan interactome in stressed vs. unstressed cells identified condensin II as a nucSF factor, suggesting their functional association in the establishment and/or maintenance of these facultative heterochromatic domains.

【 授权许可】

Unknown