Mechanochemical Preparation, Characterization and Biological Activity of Stable CuS Nanosuspension Capped by Bovine Serum Albumin

Frontiers in Chemistry

Nina Daneu¹  Erika Dutková²  Matej Baláž²  Zdenka Lukáčová Bujňáková²  Martin Kello³  Yaroslav Shpotyuk⁴  Bohdan Mahlovanyi⁴  Martin Stahorský⁵  Jelena Trajić⁶ 

[1] Advanced Materials Department, Jožef Stefan Institute, Ljubljana, Slovenia;Department of Mechanochemistry, Institute of Geotechnics, Slovak Academy of Sciences, Košice, Slovakia;Department of Pharmacology, Faculty of Medicine, P. J. Safarik University, Košice, Slovakia;Department of Sensor and Semiconductor Electronics, Ivan Franko National University of Lviv, Lviv, Ukraine;Faculty of Materials, Metallurgy and Recycling, Technical University of Košice, Košice, Slovakia;Institute of Physics, University of Belgrade, Belgrade, Serbia;Institute of Physics, University of Rzeszow, Rzeszów, Poland;

关键词: copper sulfide (CuS);    mechanochemistry;    wet stirred media milling;    capping;    bovine serum albumin (BSA);    biological activity;   

DOI  :  10.3389/fchem.2022.836795

来源: DOAJ

【 摘 要 】

The biocompatible nanosuspension of CuS nanoparticles (NPs) using bovine serum albumin (BSA) as a capping agent was prepared using a two-stage mechanochemical approach. CuS NPs were firstly synthetized by a high-energy planetary ball milling in 15 min by milling elemental precursors. The stability of nanoparticles in the simulated body fluids was studied, revealing zero copper concentration in the leachates, except simulated lung fluid (SLF, 0.015%) and simulated gastric fluid (SGF, 0.078%). Albumin sorption on CuS NPs was studied in static and dynamic modes showing a higher kinetic rate for the dynamic mode. The equilibrium state of adsorption was reached after 90 min with an adsorption capacity of 86 mg/g compared to the static mode when the capacity 59 mg/g was reached after 2 h. Then, a wet stirred media milling in a solution of BSA was introduced to yield the CuS-BSA nanosuspension, being stable for more than 10 months, as confirmed by photon cross-correlation spectroscopy. The fluorescent properties of the nanosuspension were confirmed by photoluminescence spectroscopy, which also showed that tryptophan present in the BSA could be closer to the binding site of CuS than the tyrosine residue. The biological activity was determined by in vitro tests on selected cancer and non-tumor cell lines. The results have shown that the CuS-BSA nanosuspension inhibits the metabolic activity of the cells as well as decreases their viability upon photothermal ablation.

【 授权许可】

Unknown