Rapid Determination of Major Compounds in the Ethanol Extract of Geopropolis from Malaysian Stingless Bees, Heterotrigona itama, by UHPLC-Q-TOF/MS and NMR

Molecules

Wei Cao¹  Mengjiao Yu²  Minghui Sun²  Lingling Zhao²  Xiaofeng Xue²  Liping Sun²  Tongtong Wang³ 

[1] Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, Xi’an 710069, China;Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;Institute of Quality Standard and Testing Technology for Agro–Products, Beijing 100081, China;

关键词: geopropolis;    UHPLC-Q-TOF/MS;    stingless bees;    Heterotrigona itama;   

DOI  :  10.3390/molecules22111935

来源: DOAJ

【 摘 要 】

A reliable, rapid analytical method was established for the characterization of constituents of the ethanol extract of geopropolis (EEGP) produced by Malaysian stingless bees—Heterotrigona itama—by combining ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Based on known standards, the online METLIN database, and published literature, 28 compounds were confirmed. Phenolic acids, flavones, triterpenes and phytosterol were identified or tentatively identified using characteristic diagnostic fragment ions. The results indicated that terpenoids were the main components of EEGP, accompanied by low levels of phenolic acids, flavonoids, and phytosterol. Two major components were further purified by preparative high-performance liquid chromatography (PHPLC) and identified by nuclear magnetic resonance (NMR) as 24(E)-cycloart-24-ene-26-ol-3-one and 20-hydroxy-24-dammaren-3-one. These two triterpenes, confirmed in this geopropolis for the first time, are potential chemical markers for the identification of geopropolis from Malaysian stingless bees, H. itama.

【 授权许可】

Unknown