Visualization of Aspalathin in Rooibos (Aspalathus linearis) Plant and Herbal Tea Extracts Using Thin-Layer Chromatography

Molecules

Marilize Le Roes-Hill¹  Fanie Rautenbach²  Jeanine Marnewick²  Emily Amor Stander³  Wesley Williams³  Uljana Hesse³  Yamkela Mgwatyu³ 

[1] Biocatalysis and Technical Biology Research Group, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, Symphony Road, Bellville 7535, South Africa;Oxidative Stress Research Centre, Faculty of Health and Wellness Sciences, Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, Symphony Road, Bellville 7535, South Africa;South African National Bioinformatics Institute (SANBI), University of the Western Cape, Robert Sobukwe Road, Bellville 7535, South Africa;

关键词: Rooibos;    Aspalathus linearis;    aspalathin;    thin-layer chromatography;    p-anisaldehyde;    herbal tea;    authentication;    marker compound;   

DOI  :  10.3390/molecules24050938

来源: DOAJ

【 摘 要 】

Aspalathin, the main polyphenol of rooibos (Aspalathus linearis), is associated with diverse health promoting properties of the tea. During fermentation, aspalathin is oxidized and concentrations are significantly reduced. Standardized methods for quality control of rooibos products do not investigate aspalathin, since current techniques of aspalathin detection require expensive equipment and expertise. Here, we describe a simple and fast thin-layer chromatography (TLC) method that can reproducibly visualize aspalathin in rooibos herbal tea and plant extracts at a limit of detection (LOD) equal to 178.7 ng and a limit of quantification (LOQ) equal to 541.6 ng. Aspalathin is a rare compound, so far only found in A. linearis and its (rare) sister species A. pendula. Therefore, aspalathin could serve as a marker compound for authentication and quality control of rooibos products, and the described TLC method represents a cost-effective approach for high-throughput screening of plant and herbal tea extracts.

【 授权许可】

Unknown