FaHSP17.8-CII orchestrates lead tolerance and accumulation in shoots via enhancing antioxidant enzymatic response and PSII activity in tall fescue

Ecotoxicology and Environmental Safety

Huawei Xu¹  Guangyang Wang¹  Maurice Amee²  Tao Hu²  Tao Wang³  Yan Xie⁴ 

[1] Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan 430074, China;CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China;College of Agriculture, Henan University of Science and Technology, Luoyang, China;School of Resources and Environmental Engineering, Ludong University, Yantai, China;

关键词: Lead pollution;    Phytoremediation;    Tall fescue;    Photosystem II;    Genetic modification;   

DOI  :  

来源: DOAJ

【 摘 要 】

Tall fescue (Festuca arundinacea Schreb.) shows huge potential for lead (Pb) phytoremediation, while little is known on the molecular mechanisms involved in Pb tolerance and accumulation. Here, genetic engineering strategy was firstly used to investigate Pb tolerance and accumulation in tall fescue. The transgenic tall fescue overexpressing a class II (CII) sHSP gene FaHSP17.8-CII was generated. After exposure to 1000 mg/L Pb(NO3)2, two FaHSP17.8-CII overexpressing lines, OE#3 and OE#7, showed higher tolerance to Pb as illustrated by the reduced levels of electrolyte leakage (EL) and malondialdehyde (MDA) as compared to the wild-type (WT) plants under Pb stress. Moreover, the FaHSP17.8-CII overexpression lines, OE#3 and OE#7, exhibited 36.3% and 46.6% higher shoot Pb accumulation relative to the WT grasses. When the grasses were exposed to Pb stress, the two OE lines had higher CAT, POD and SOD activities as compared to WT. Additionally, overexpression of FaHSP17.8-CII improved the synthesis of chlorophyll and transcript abundance of FapsbC, FapsbD and FapsbE, and alleviated the photoinhibition of PSII in tall fescue under Pb stress. This study provides an initial genetic engineering strategy to improve Pb phytoremediation efficiency in tall fescue by FaHSP17.8-CII overexpression.

【 授权许可】

Unknown