Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches

Genome Biology

Douwe van Sinderen¹  Maria Cristina Ossiprandi²  Giulia Alessandri²  Francesca Turroni³  Sabrina Duranti³  Gabriele Andrea Lugli³  Marco Ventura³  Christian Milani³  Danilo Tatoni³  Leonardo Mancabelli³ 

[1] APC Microbiome Institute and School of Microbiology, Bioscience Institute, National University of Ireland;Department of Veterinary Medical Science, University of Parma;Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma;

关键词: Genomics;    Metagenomics;    Microbiota;    Human gut commensals;   

DOI  :  10.1186/s13059-019-1711-6

来源: DOAJ

【 摘 要 】

Abstract Whole metagenome shotgun (WMGS) sequencing is a method that provides insights into the genomic composition and arrangement of complex microbial consortia. Here, we report how WMGS coupled with a cultivation approach allows the isolation of novel bifidobacteria from animal fecal samples. A combination of in silico analyses based on nucleotide and protein sequences facilitate the identification of genetic material belonging to putative novel species. Consequently, the prediction of metabolic properties by in silico analyses permits the identification of specific substrates that are then employed to isolate these species through a cultivation method.

【 授权许可】

Unknown