Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents

Nanomaterials

Anaëlle Torres¹  Bastien Dalzon¹  Thierry Rabilloud¹  Marie-Edith Arnal²  Marie Carriere²  Fanny Dussert²  Pierre-Adrien Arthaud²  Thierry Douki²  Nathalie Herlin³ 

[1] Chemistry and Biology of Metals, Université Grenoble Alpes, CNRS UMR5249, CEA, IRIG-DIESE-LCBM-ProMD, F-38054 Grenoble, France;Université Grenoble-Alpes, CEA, CNRS, IRIG-DIESE, SyMMES, Chemistry Interface Biology for the Environment, Health and Toxicology (CIBEST), F-38000 Grenoble, France;Université Paris Saclay, CEA Saclay, IRAMIS NIMBE UMR 3685, 91191 Gif/Yvette CEDEX, France;

关键词: silica;    SiO₂;    nanoparticle;    E551;    toxicity;    genotoxicity;   

DOI  :  10.3390/nano10071418

来源: DOAJ

【 摘 要 】

Synthetic amorphous silica (SAS) is used in a plethora of applications and included in many daily products to which humans are exposed via inhalation, ingestion, or skin contact. This poses the question of their potential toxicity, particularly towards macrophages, which show specific sensitivity to this material. SAS represents an ideal candidate for the adsorption of environmental contaminants due to its large surface area and could consequently modulate their toxicity. In this study, we assessed the toxicity towards macrophages and intestinal epithelial cells of three SAS particles, either isolated SiO₂ nanoparticles (LS30) or SiO₂ particles composed of agglomerated-aggregates of fused primary particles, either food-grade (E551) or non-food-grade (Fumed silica). These particles were applied to cells either alone or in combination with genotoxic co-contaminants, i.e., benzo[a]pyrene (B[a]P) and methane methylsulfonate (MMS). We show that macrophages are much more sensitive to these toxic agents than a non-differenciated co-culture of Caco-2 and HT29-MTX cells, used here as a model of intestinal epithelium. Co-exposure to SiO₂ and MMS causes DNA damage in a synergistic way, which is not explained by the modulation of DNA repair protein mRNA expression. Together, this suggests that SiO₂ particles could adsorb genotoxic agents on their surface and, consequently, increase their DNA damaging potential.

【 授权许可】

Unknown