Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of OvineArticular Cartilage

International Journal of Molecular Sciences

Alessandro R. Zorzi¹  João B. Miranda¹  Eliane M. I. Amstalden²  Angela C. M. Luzo³  Adriana S. S. Duarte³  Eliane Antonioli⁴  Mario Ferretti⁴  Virginia C. A. Martins⁵  Ana Maria G. Plepis⁵ 

[1] Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil;Department of Pathology, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil;Hematology Hemotherapy Centre, Universidade de Campinas (UNICAMP), Campinas/SP 13083-878, Brazil;Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil;Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos/SP 13566-590, Brazil;

关键词: tissue engineering;    regenerative medicine;    adult stem cell;    stem cell therapy;    biomaterials;    cartilage;   

DOI  :  10.3390/ijms161125989

来源: DOAJ

【 摘 要 】

Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score(8.3 ± 3.1), followed by the SCAFFOLD group (5.6 ± 2.2) and the EMPTY group (5.2 ± 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model.

【 授权许可】

Unknown