Effect of 3-subsitution of quinolinehydroxamic acids on selectivity of histone deacetylase isoforms

Journal of Enzyme Inhibition and Medicinal Chemistry

Mei-Jung Lai¹  Yuh-Hsuan Chao²  Mei-Chuan Chen²  Jing-Ping Liou²  Hsueh-Yun Lee²  Samir Mehndiratta²  Cheng-Hsin Lee² 

[1] Biomedical Commercialization Center, Taipei Medical University;School of Pharmacy, College of Pharmacy, Taipei Medical University;

关键词: quinoline;    hdac;    lung cancer;    colon cancer;    hydroxamic acid;    acrylamide;   

DOI  :  10.1080/14756366.2020.1839446

来源: DOAJ

【 摘 要 】

A series of 3-subsituted quinolinehydroxamic acids has been synthesised and evaluated for their effect on human lung cancer cell line (A549), human colorectal cancer cell line (HCT116) and HDAC isoforms 1, 2, 6, and 8. The results indicated that substitution at C3 of quinoline is favoured for HDAC6 selectivity. Two compounds (25 and 26) were also found to be potent anti-proliferative compounds with IC50 values ranging from 1.29 to 2.13 µM against A549 and HCT116 cells. These compounds displayed remarkable selectivity for HDAC6 over other HDAC isoforms with nanomolar IC50 values. Western blot analysis revealed that compounds of this series activate apoptotic caspase pathway as indicated by cleavage of caspase 3, 8, and 9 and also increase phosphorylated H2AX thus inducing DNA double strand fragmentation in a concentration dependent manner. Flow cytometric analysis also displayed a dose dependent increase of cell population in sub G1 phase.

【 授权许可】

Unknown