Exosome Processing and Characterization Approaches for Research and Technology Development

Advanced Science

James J. Lai¹  John J. Hill¹  Katalin V. Korpany¹  Joanne K. Liu¹  Zoe L. Chau¹  Ruby Lunde¹  Wei‐Ting Shen²  Yi‐Hsuan Lin³  Yu‐Chung Liu⁴  Nai‐Wen Liang⁴  Li‐Han Lin⁵  Sheng‐You Chen⁶ 

[1]Department of Bioengineering University of Washington Seattle WA 98195 USA

[2]Department of Biomedical Engineering and Environmental Sciences National Tsing Hua University Hsinchu 30013 Taiwan

[3]Department of Engineering and System Science National Tsing Hua University Hsinchu 30013 Taiwan

[4]Department of Materials Science and Engineering National Tsing Hua University Hsinchu 30013 Taiwan

[5]Department of Mechanical Engineering National Taiwan University Taipei City 10617 Taiwan

[6]Department of Mechanical Engineering University of Washington Seattle WA 98195 USA

关键词: analytical characterizations;    exosomes;    exosome clinical trials;    extracellular vesicles;    Good Manufacturing Practices;    isolation processes;   

DOI  :  10.1002/advs.202103222

来源: DOAJ

【 摘 要 】

Abstract Exosomes are extracellular vesicles that share components of their parent cells and are attractive in biotechnology and biomedical research as potential disease biomarkers as well as therapeutic agents. Crucial to realizing this potential is the ability to manufacture high‐quality exosomes; however, unlike biologics such as proteins, exosomes lack standardized Good Manufacturing Practices for their processing and characterization. Furthermore, there is a lack of well‐characterized reference exosome materials to aid in selection of methods for exosome isolation, purification, and analysis. This review informs exosome research and technology development by comparing exosome processing and characterization methods and recommending exosome workflows. This review also provides a detailed introduction to exosomes, including their physical and chemical properties, roles in normal biological processes and in disease progression, and summarizes some of the on‐going clinical trials.

【 授权许可】

Unknown