Antioxidative defense mechanism of the ruderal Verbascum olympicum Boiss. against copper (Cu)-induced stress

Open Life Sciences

Arslan Hülya¹  Güleryüz Gürcan¹  Akpınar Ayşegül Uğar¹  Kırmızı Serap² 

[1] Department of Biology, Faculty of Art and Sciences, University of Uludağ16059, Görökle-Bursa, Turkey;Gemlik Asim Kocabiyik Graduate Vocational School, University of Uludağ16059, Gemlik-Bursa, Turkey;

关键词: copper;    superoxide dismutase;    ascorbate peroxidase;    catalase;    v. olympicum;   

DOI  :  10.1515/biol-2016-0002

来源: DOAJ

【 摘 要 】

The endemic Verbascum olympicum has characteristics that allows it to live in degraded areas of Uludağ Mountain, Turkey and therefore known as a ruderal species. In this study, V. olympicum seeds collected from Uludağ Mountain were grown in the Hoagland nutrient solution, under hydroponic conditions. The activity of antioxidative enzymes (superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX) were examined to demonstrate the role of antioxidative mechanism in the seedlings exposed to different Cu concentrations (0, 50, 250, 500 μM) for seven days. Also, certain growth parameters (such as the water content, biomass production, soluble protein), the level of lipid peroxidation, cell membrane injury and permeability were investigated. As a result, some toxic effects are observed following the application of 500 μM Cu, after the seedlings growing in 50 and 250 mM Cu concentrations showed high resistance and survived in hydroponic conditions. Our findings provide information about the resistance of V. Olympicum seedlings to oxidative stress caused by excessive Cu concentrations.

【 授权许可】

Unknown