Antioxidant and Antihypertensive Effects of a Chemically Defined Fraction of Syrah Red Wine on Spontaneously Hypertensive Rats

Nutrients

Naiane Ferraz Bandeira Alves¹  Clenia de Oliveira Cavalcanti¹  Eugênia Abrantes de Figueiredo¹  Matheus Morais de Oliveira Monteiro¹  Valdir de Andrade Braga¹  Eduardo de Jesus Oliveira²  Tania Maria Sarmento da Silva³  Telma Maria Guedes da Silva³ 

[1] Centro de Biotecnologia, Programa de Pós Graduação em Produtos Naturais e Sintéticos Bioativos, Campus I, João Pessoa, PB 58051-970, Brazil;Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucurí, Diamantina, MG, 39100-000, Brazil;Departamento de Química, Laboratório de Bioprospecção Fitoquímica, Universidade Federal Rural de Pernambuco, Recife, PE 52171-900, Brazil;

关键词: red wine;    phenolics;    hypertension;    flavonoids;   

DOI  :  10.3390/nu9060574

来源: DOAJ

【 摘 要 】

A particularly phenolic-rich fraction extracted from red wine from the São Francisco valley (Northeastern Brazil) was chemically characterized and its hypotensive and antioxidant effects on spontaneously hypertensive rats were studied both in vitro and in vivo. The liquid-liquid pH dependent fractionation scheme afforded a fraction with high content of bioactive phenolics such as flavonols, flavonol glycosides, phenolic acids and anthocyanins, whose identities were confirmed by liquid chromatography coupled to mass spectrometry analysis. Pretreatment of spontaneously hypertensive rats with this wine fraction at doses of 50 and 100 mg/kg by gavage. for 15 days was able to decrease mean arterial pressure and heart rate as well as decrease serum lipid peroxidation. The fraction at concentrations of 0.01–1000 µg/mL induced concentration-dependent relaxation of isolated rat superior mesenteric artery rings pre-contracted with phenylephrine and this effect was not attenuated by endothelium removal. Our results demonstrate it is possible for phenolic constituents of red wine that are orally bioavailable to exert in vivo hypotensive and antioxidant effects on intact endothelial function.

【 授权许可】

Unknown