Heterobasidion annosum Induces Apoptosis in DLD-1 Cells and Decreases Colon Cancer Growth in In Vivo Model

International Journal of Molecular Sciences

Magdalena Jaszek¹  Anna Pawlik¹  Katarzyna Niemirowicz-Laskowska²  Halina Car²  Diana Sawicka²  Anna Sadowska²  Arkadiusz Surażyński³  Katarzyna Sułkowska-Ziaja⁴  Katarzyna Kała⁴  Bożena Muszyńska⁴  Marcin Stocki⁵  Ewa Zapora⁵  Sławomir Bakier⁵  Marek Wołkowycki⁵ 

[1] Department of Biochemistry and Biotechnology, Maria Curie Sklodowska University, Akademicka 19, 20-033 Lublin, Poland;Department of Experimental Pharmacology, Medical University of Białystok, Szpitalna 37, 15-295 Bialystok, Poland;Department of Medicinal Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland;Department of Pharmaceutical Botany, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland;Institute of Forest Sciences, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland;

关键词: Heterobasidion annosum;    colon cancer;    apoptosis;    active substances;    mouse model;    toxicity;   

DOI  :  10.3390/ijms21103447

来源: DOAJ

【 摘 要 】

Application of substances from medicinal mushrooms is one of the interesting approaches to improve cancer therapy. In this study, we commenced a new attempt in the field of Heterobasidion annosum (Fr.) Bref. sensu lato to further extend our knowledge on this basidiomycete fungus. For this purpose, analysis of the active substances of Heterobasidion annosum methanolic extract and also its influence on colorectal cancer in terms of in vitro and in vivo experiments were performed. In vivo studies on mice were conducted to verify its acute toxicity and to further affirm its anticancer potential. Results indicated that all the most common substances of best known medicinal mushrooms that are also responsible for their biological activity are present in tested extracts. In vitro tests showed a high hemocompatibility and a significant decrease in viability and proliferation of DLD-1 cells in a concentration-dependent manner of Heterobasidion annosum extract. The studies performed on xenograft model of mice showed lower tendency of tumor growth in the group of mice receiving Heterobasidion annosum extract as well as mild or moderate toxicity. Obtained results suggest beneficial potential of Heterobasidion annosum against colon cancer as cytotoxic agent or as adjuvant anticancer therapy.

【 授权许可】

Unknown