Investigation of adipose tissues in Zucker rats using in vivo and ex vivo magnetic resonance spectroscopy

Journal of Lipid Research

Marco Fontanella¹  Silvia Fiorini²  Sabine Van Huffel²  Pasquina Marzola³  Diana M. Sima³  Andrea Sbarbati³  Elisa Mosconi⁴ 

[1] Magnetic Resonance Laboratory, University of Verona, Verona, Italy;Department of Electrical Engineering (ESAT-SCD), Katholieke Universiteit Leuven, Leuven, Belgium;Magnetic Resonance Laboratory, University of Verona, Verona, Italy;To whom correspondence should be addressed.;

关键词: oil;    fat;    obese Zucker;    unsaturation;   

DOI  :  

来源: DOAJ

【 摘 要 】

In vivo single-voxel magnetic resonance spectroscopy (MRS) at 4.7T and ex vivo high-resolution proton magnetic resonance spectroscopy (HR-NMR) at 500 MHz were used to study the composition of adipose tissues in Zucker obese and Zucker lean rats. Lipid composition was characterized by unsaturation and polyunsaturation indexes and mean chain lengths. In vitro experiments were conducted in known mixtures of triglycerides and oils in order to validate the method. To avoid inaccuracies due to partial peak overlapping in MRS, peak quantification was performed after fitting of spectral peaks by using the QUEST algorithm. The intensity of different spectral lines was also corrected for T2 relaxation. Albeit with different sensitivity and accuracy, both techniques revealed that white adipose tissue is characterized by lower unsaturation and polyunsaturation indexes in obese rats compared with controls. HR-NMR revealed similar differences in brown adipose tissue. The present findings confirm the hypothesis that obese and lean Zucker rats have different adipose tissue composition.

【 授权许可】

Unknown