Annealing dependent synthesis of cyto-compatible nano-silver/calcium hydroxyapatite composite for antimicrobial activities

Arabian Journal of Chemistry

Gunasekaran Suriyakala¹  K. Kaviyarasu¹  Rajendran Velmurugan²  Yang Zang³  Arumugam Dhanesh Gandhi⁴  Tse-Wei Chen⁴  Khalid S. Almaary⁵  Khantong Soontarapa⁵  Ranganathan Babujanarthanam⁶  Mohamed S. Elshikh⁷  Nookala Supraja⁸ 

[1] Corresponding authors.;Nanosciences African Network (NANOAFNET), Materials Research Group (MRG), IThemba LABS-National Research Foundation (NRF), 1 Old Faure Road, 7129, PO Box 722, Somerset West, Western Cape Province, South Africa;Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;Nano and Energy Biosciences Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India;Nanotechnology Laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University, Tirupati 517 502, AP, India;Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Science (CAS), China;UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology Laboratories, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa;

关键词: Hydroxyapatite;    Ureolytic Bacillus sp.;    Synthetic urine;    Antibacterial activity;    Anticancer studies;    Brine shrimp assay;   

DOI  :  

来源: DOAJ

【 摘 要 】

Crystalline hydroxyapatite were synthesized from synthetic/human urine through precipitation which were further doped with silver nanoparticle for effective biomedical application. The aim were to improve overall biological compatibility of the synthesized bone-graft material even in oncogenesis cases. The thermal calcinated material was characterized by several techniques including UV–vis, Laser Raman, Fourier transmittance infrared spectroscopy, X-ray diffraction analysis, Transmission Eelectron microscopy and X-ray fluorescence spectroscopy. The quantitative and qualitative analysis revealed that the synthesized material was highly crystalline and nanosized with majority of silver and phosphate components. The antibacterial, anticancer and invitro cytotoxicity of the synthesized material was evaluated with Escherichia coli, Hela cells and brine shrimp assay, respectively. The brine shrimp assay revealed that the synthesized material is compatible with biological system, whereas anticancer activity showed the application of the synthesized biomaterial in cancer treatment in which antibacterial activity adds more advantage on preventing the bone-graft from microbial attack.

【 授权许可】

Unknown