Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Journal of Oral Research

María Correnti¹  Zoraya De Guglielmo²  Dayahindara Veitía²  Juan Liuzzi³  Maira Ávila³ 

[1] Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología de la UCV, Caracas, Venezuela.;Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas, Venezuela.;Servicio de cabeza y cuello del Hospital Oncológico ‘Padre Machado’-IVSS, Caracas, Venezuela.;

关键词: Carcinoma;    VPH;    VEB;    p53;    PCNA.;   

DOI  :  10.17126/joralres.2017.026

来源: DOAJ

【 摘 要 】

Introduction: Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions. Objective: To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma. Methodology: Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining. Results: The mean age of the patients was 60.55±13.94 years, and 52% of these were female. Of the patients, 59% were tobacco users and 63% were alcohol consumers. HPV was detected in 70% of the patients with the predominance of genotype 16 (60%). As for EBV infection, it was observed in 59% of cases. p53 and PCNA immunopositivity corresponded to 44% and 59%, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48% of the cases presented infection by both viruses. Conclusion: HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.

【 授权许可】

Unknown