Variable in Vivo and in Vitro Biological Effects of Cerium Oxide Nanoparticle Formulations

Frontiers in Pharmacology

Joseph Chiarenzelli¹  Stephanie Vangellow¹  Bonnie Hays-Erlichman¹  Joseph S. Erlichman¹  Samantha Ribeiro¹  Karin L. Heckman¹  William DeCoteau²  Ana Y. Estevez² 

[1] Department of Biology, St. Lawrence University, Canton, NY, United States;Department of Psychology, St. Lawrence University, Canton, NY, United States;

关键词: cerium oxide nanoparticles;    oxidative stress;    antioxidants;    experimental autoimmune encephalomyelitis;    macrophages;   

DOI  :  10.3389/fphar.2019.01599

来源: DOAJ

【 摘 要 】

Cerium oxide nanoparticles (CeNPs) exhibit redox capacity in vitro with efficacy in in vivo disease models of oxidative stress. Here we compare, in parallel, three CeNP formulations with distinct chemical stabilizers and size. In vitro assays revealed antioxidant activity from all the CeNPs, but when administered to mice with a reactive oxygen species (ROS) mediated model of multiple sclerosis, only custom-synthesized Cerion NRx (CNRx) citrate-EDTA stabilized CeNPs provided protection against disease. Detectable levels of ceria and reduced ROS levels in the brains of CNRx CeNP-treated mice imply that these CeNPs' unique properties influence tissue distribution and subsequent biological activity, suggesting why differing CeNP formulations yield different in vivo effects in various models. Further, the variation in in vivo vs in vitro results with these CeNP formulations highlights the necessity for in vivo studies that confirm whether the inherent catalytic activity of CeNPs is maintained after transport and distribution within intact biological systems.

【 授权许可】

Unknown