Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons

Frontiers in Cellular Neuroscience

Eddie Grinman¹  Supriya Swarnkar¹  Sathyanarayanan V. Puthanveettil¹  Nadine F. Joseph¹ 

[1] Department of Neuroscience, The Scripps Research Institute, Jupiter, FL, United States;The Skaggs Graduate School of Chemical and Biological Sciences, The Scripps Research Institute, La Jolla, CA, United States;

关键词: kinesins;    KIF3B;    dendritic arborization;    spine density;    spine morphology;    structural plasticity;   

DOI  :  10.3389/fncel.2020.521199

来源: DOAJ

【 摘 要 】

Neurons require a well-coordinated intercellular transport system to maintain their normal cellular function and morphology. The kinesin family of proteins (KIFs) fills this role by regulating the transport of a diverse array of cargos in post-mitotic cells. On the other hand, in mitotic cells, KIFs facilitate the fidelity of the cellular division machinery. Though certain mitotic KIFs function in post-mitotic neurons, little is known about them. We studied the role of a mitotic KIF (KIF3B) in neuronal architecture. We find that the RNAi mediated knockdown of KIF3B in primary cortical neurons resulted in an increase in spine density; the number of thin and mushroom spines; and dendritic branching. Consistent with the change in spine density, we observed a specific increase in the distribution of the excitatory post-synaptic protein, PSD-95 in KIF3B knockdown neurons. Interestingly, overexpression of KIF3B produced a reduction in spine density, in particular mushroom spines, and a decrease in dendritic branching. These studies suggest that KIF3B is a key determinant of cortical neuron morphology and that it functions as an inhibitory constraint on structural plasticity, further illuminating the significance of mitotic KIFs in post-mitotic neurons.

【 授权许可】

Unknown