Italian Journal of Animal Science | |
Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species | |
Jayme Aparecido Povh1  Claudete de Fátima Ruas2  Renata Mussoi Giacomin2  Bruna Delgado Goes2  Nelson Mauricio Lopera-Barrero2  Felipe Pinheiro de Souza2  Angela Maria Urrea-Rojas2  Eduardo Augusto Ruas2  Ricardo Pereira Ribeiro3  Pedro Luiz de Castro3  | |
[1] Federal University of Mato Grosso do Sul;State University of Londrina;State University of Maringá; | |
关键词: Genetic conservation; genetic diversity; molecular marker; Piracanjuba; SSR; | |
DOI : 10.1080/1828051X.2018.1436008 | |
来源: DOAJ |
【 摘 要 】
Brycon orbignyanus is a neotropical fish found in several South American countries. This species has high ecological importance and is included in the list of fish threatened with extinction. In this study, we report the development and characterisation of 12 microsatellite primers of B. orbignyanus for future genetic studies. Screening of the 12 markers in a sample of 35 individuals identified seven polymorphic loci and 22 alleles, with one to three alleles per locus. The polymorphic information content ranged from 0.054 to 0.542, with four loci showing significant deviation from Hardy–Weinberg equilibrium. Two loci possessed null alleles. Cross-species amplification tests in two closely related species showed that 11 loci are transferrable to Brycon gouldingi (B. gouldingi) and nine are transferrable to B. falcatus. The microsatellite primers designed may be used for genetic variability and population structure studies in wild populations, fish farms and restocking programmes of B. orbignyanus and the two related species.
【 授权许可】
Unknown