Are Silver Nanoparticles Useful for Treating Second-Degree Burns? An Experimental Study in Rats

Advanced Pharmaceutical Bulletin

Stephen Hyslop¹  Valquíria Miwa Hanai-Yoshida²  Murilo Melo Juste Dini³  Fabia Judice Marquesudice Viroel³  Denise Grotto³  Carolina Alves dos Santos³  Débora Antunes Neto Moreno³  Yoko Oshima-Franco³  Thaisa Borim Pickler⁴  Marli Gerenutti⁵  Jorge Amaral Filho⁶  Mirela Santos Saladini⁶ 

[1] Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.;Graduate Program in Environmental and Technological Processes, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.;Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.;Laboratory for Toxicological Research (Lapetox), University of Sorocaba (UNISO), Sorocaba, SP, Brazil.;School of Medical Sciences of the Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, SP, Brazil.;Veterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.;

关键词: hematological parameters;    oxidative stress;    second-degree burn;    silver nanoparticles;    wound healing;   

DOI  :  10.34172/apb.2021.014

来源: DOAJ

【 摘 要 】

Purpose: In this work, the potential usefulness of silver nanoparticles (AgNPs) for treating burn wounds was examined. Methods: Second-degree burns were induced in male Wistar rats by touching the skin with a heated (70°C) metallic device for 10 s, after which the animals were randomly allocated to one of two groups: control (n=8, treated with sterile saline) and experimental (n=8, treated with AgNPs, 0.081 mg/mL; 50 µL applied to the burn surface). Seven, 14, 21 and 28 days after lesion induction two rats from each group were killed and blood samples were collected for a complete blood count and to assess oxidative stress. The livers were examined macroscopically and skin samples were collected for histological analysis. Results: Macroscopically, wound healing and skin remodeling in the experimental group were similar to the saline-treated rats. Likewise, there were no significant differences in the histological parameters between the two groups. However, treatment with AgNPs caused a persistent reduction in white blood cell (WBC) counts throughout the experiment, whereas platelet counts increased on days 7 and 28 but decreased on days 14 and 21; there was also an increase in the blood concentration of reduced glutathione on day 7 followed by a decrease on days 21 and 28. There were no significant changes in blood glutathione peroxidase (GSH-Px) and catalase (CAT) activities or in the serum concentration of thiobarbituric acid reactive substances. Conclusion: The findings of this study raise questions about the potential transitory effects of AgNPs based on the changes in WBC and platelet counts, blood glutathione concentrations and macroscopic hepatic alterations.

【 授权许可】

Unknown