Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut

Insects

Li Chen¹  Wenkai Wang²  Guanglin Xie²  Nuo Zhang²  Mengmeng Zhang²  Zhihao Cui² 

[1] Institute of Life Science and Green Development, College of Life Science, Hebei University, Baoding 071002, China;School of Agriculture, Yangtze University, Jingzhou 434025, China;

关键词: plant volatiles;    dark black chafer;    GC-EAD;    host location;    field trapping;    hexanal;   

DOI  :  10.3390/insects12020158

来源: DOAJ

【 摘 要 】

Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae) is a notorious pest of many crops, especially peanuts. In this study, volatiles from peanut plants were analyzed using both gas chromatographic-electroantennographic detection (GC-EAD) and gas chromatography/mass spectrometry (GC/MS) techniques, and tested for adult attraction with field trapping bioassays in Hebei Province, China. GC-EAD analyses indicated that H. parallela antennae strongly responded to twelve GC peaks, including eight identified compounds, (Z)-β-ocimene, hexanal, 6-methyl-5-hepten-2-one, nonanal, dihydromyrcenol, linalool, β-caryophyllene, methyl salicylate, and four unidentified compounds. When tested individually in field conditions from 24 to 31 July, 2020, β-caryophyllene and hexanal significantly attracted both sexes of H. parallela, whereas all other compounds were unattractive. A blend of β-caryophyllene and hexanal at a ratio of 2:1, close to the natural ratio of these two compounds from the intact peanut plant, was most attractive to the beetles. The remaining identified compounds, (Z)-β-ocimene, 6-methyl-5-hepten-2-one, nonanal, dihydromyrcenol, linalool, and methyl salicylate had no synergistic effects on H. parallela attraction when tested in combination with the blend of β-caryophyllene and hexanal. These results demonstrated that β-caryophyllene and hexanal in the volatiles from peanut plants have strong attraction to H. parallela. These two compounds have the potential to be used for monitoring H. parallela and its management programs.

【 授权许可】

Unknown