Lipopolysaccharide from Crypt-Specific Core Microbiota Modulates the Colonic Epithelial Proliferation-to-Differentiation Balance

mBio

Amy B. Pedersen¹  Thierry Pédron¹  Marion Bérard²  Cristina De Castro³  Antonio Molinaro⁴  Mélanie Clerc⁵  Philippe J. Sansonetti⁵  Azadeh Saffarian⁶  Giulia Nigro⁶  Tomoaki Naito⁶  Céline Mulet⁶ 

[1] Centre for Immunity, Infection and Evolution (CIIE), University of Edinburgh, Edinburgh, United Kingdom;Animalerie Centrale, Institut Pasteur, Paris, France;Department of Agricultural Sciences, Porici (NA), Italy;Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Naples, Italy;;School of Biological Sciences &Unité de Pathogénie Microbienne Moléculaire, INSERM U1202, Institut Pasteur, Paris, France;

关键词: homeostasis;    intestinal stem cells;    LPS;    necroptosis;   

DOI  :  10.1128/mBio.01680-17

来源: DOAJ

【 摘 要 】

ABSTRACT We identified a crypt-specific core microbiota (CSCM) dominated by strictly aerobic, nonfermentative bacteria in murine cecal and proximal colonic (PC) crypts and hypothesized that, among its possible functions, it may affect epithelial regeneration. In the present work, we isolated representative CSCM strains using selective media based upon our initial 16S rRNA-based molecular identification (i.e., Acinetobacter, Delftia, and Stenotrophomonas). Their tropism for the crypt was confirmed, and their influence on epithelial regeneration was demonstrated in vivo by monocolonization of germfree mice. We also showed that lipopolysaccharide (LPS), through its endotoxin activity, was the dominant bacterial agonist controlling proliferation. The relevant molecular mechanisms were analyzed using colonic crypt-derived organoids exposed to bacterial sonicates or highly purified LPS as agonists. We identified a Toll-like receptor 4 (TLR4)-dependent program affecting crypts at different stages of epithelial differentiation. LPS played a dual role: it repressed cell proliferation through RIPK3-mediated necroptosis of stem cells and cells of the transit-amplifying compartment and concurrently enhanced cell differentiation, particularly the goblet cell lineage. IMPORTANCE The LPS from crypt-specific core microbiota controls intestinal epithelium proliferation through necroptosis of stem cells and enhances cell differentiation, mainly the goblet cell lineage.

【 授权许可】

Unknown