Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area

Revista da Sociedade Brasileira de Medicina Tropical

Marta Cristhiany Cunha Pinheiro¹  Ana Lúcia de Paula Hanemann¹  Teiliane Rodrigues Carneiro¹  José Ajax Nogueira Queiroz¹  Sara Menezes de Oliveira¹  Fernando Schemelzer Moraes Bezerra¹ 

[1] Universidade Federal do Ceará;

关键词: Esquistossomose;    Diagnóstico;    Schistosoma mansoni;    Kato-Katz;    ELISA;   

DOI  :  10.1590/S0037-86822012000400019

来源: DOAJ

【 摘 要 】

INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique.

【 授权许可】

Unknown