In Vitro Monitoring of Magnesium-Based Implants Degradation by Surface Analysis and Optical Spectroscopy

International Journal of Molecular Sciences

Alejandro Barrantes¹  Maryam Rahmati¹  Håvard Jostein Haugen¹  Hafiz Wajahat Hassan²  Peyman Mirtaheri² 

[1] Department of Biomaterials, Institute of Clinical Dentistry and Oral Research Laboratory, University of Oslo, 0317 Oslo, Norway;Department of Mechanical, Electronic and Chemical Engineering, Faculty of Technology, Art and Design, Oslo Metropolitan University, 0130 Oslo, Norway;

关键词: degradation of magnesium;    optical spectroscopy;    partial-least-squares regression;    bubble formation;    in vitro monitoring;   

DOI  :  10.3390/ijms23116099

来源: DOAJ

【 摘 要 】

Magnesium (Mg)-based degradable alloys have attracted substantial attention for tissue engineering applications due to their biodegradability and potential for avoiding secondary removal surgeries. However, insufficient data in the existing literature regarding Mg’s corrosion and gas formation after implantation have delayed its wide clinical application. Since the surface properties of degradable materials constantly change after contact with body fluid, monitoring the behaviour of Mg in phantoms or buffer solutions could provide some information about its physicochemical surface changes over time. Through surface analysis and spectroscopic analysis, we aimed to investigate the structural and functional properties of degradable disks. Since bubble formation may lead to inflammation and change pH, monitoring components related to acidosis near the cells is essential. To study the bubble formation in cell culture media, we used a newly developed Mg alloy (based on Mg, zinc, and calcium), pure Mg, and commercially available grade 2 Titanium (Ti) disks in Dulbecco’s Modified Eagle Medium (DMEM) solution to observe their behaviour over ten days of immersion. Using surface analysis and the information from near-infrared spectroscopy (NIRS), we concluded on the conditions associated with the medical risks of Mg alloy disintegration. NIRS is used to investigate the degradation behaviour of Mg-based disks in the cell culture media, which is correlated with the surface analysis where possible.

【 授权许可】

Unknown