Dataset on recombinant expression of an ancient chitinase gene from different species of Leishmania parasites in bacteria and in Spodoptera frugiperda cells using baculovirus

Data in Brief

Tanil Lacerda Góis Filho¹  Renata Torres da Costa²  Rodrigo Buzinaro Suzuki³  Edmar Silva Santos³  Aline Diniz Cabral³  Ligia Marinho Pereira Vasconcelos³  Márcia Aparecida Sperança³  Felipe Baena Garcia³ 

[1] Department of Parasitology, Marília Medical School, 17519-030 Marília, SP, Brasil;School of Medicine, University of Marilia, 17.525-902 Marília, SP, Brazil;Center for Natural and Human Sciences, Universidade Federal do ABC, Rua Arcturus, 03 - Bloco Delta, Sala 226, Laboratório 107, 09606-070 São Bernardo do Campo, SP, Brazil;

关键词: Leishmania sp;    Chitinase;    Baculoviruses;    Sf9 insect cell;    Recombinant protein;   

DOI  :  

来源: DOAJ

【 摘 要 】

The data presented here is related to negative results obtained with the recombinant expression of chitinase from four species of Leishmania parasites in two expression systems, performed in order to investigate the molecular characteristics of the Leishmania chitinase and its possible application in leishmaniasis diagnosis. Thus, heterologous Leishmania sp chitinase proteins were expressed in bacteria using the prokaryotic expression vector pET28a and Escherichia coli Mach-T1, and in Spodoptera frugiperda (Sf9) insect cells, using the eukaryotic bac-to-bac expression system (Thermo Fisher Scientific) to produce recombinant baculoviruses to infect Sf9. Biochemical and cellular analysis of the various recombinant forms of the Leishmania sp chitinase produced in prokaryotic and eukaryotic expression systems were performed through SDS-PAGE and Western blotting. Chitinase produced and purified from bacteria presented low yield and formed inactive aggregates. Heterologous chitinase obtained after infection of Sf9 insect cells with all the four Leishmania species recombinant baculoviruses presented high yield of insoluble proteins. Dot-blot serological tests presented inconclusive results against the recombinant Leishmania sp chitinases produced in both expression systems. The experiments described in this paper can help researchers to avoid errors when choosing a recombinant expression systems to produce Leishmania parasites proteins for biotechnological purposes.

【 授权许可】

Unknown