The SF3B1 R625H mutation promotes prolactinoma tumor progression through aberrant splicing of DLG1

Journal of Experimental & Clinical Cancer Research

Qiuyue Fang¹  Yulou Liu¹  Weiyan Xie¹  Yiyuan Chen¹  Jing Guo¹  Dawei Wang¹  Yazhuo Zhang¹  Chuzhong Li² 

[1] Beijing Neurosurgical Institute, Capital Medical University;Department of Neurosurgery, Beijing Tiantan Hospital affiliated to Capital Medical University;

关键词: SF3B1 mutation;    Prolactinomas;    Alternative splicing;    DLG1;    Invasion;   

DOI  :  10.1186/s13046-022-02245-0

来源: DOAJ

【 摘 要 】

Abstract Background Recently, a hotspot mutation in prolactinoma was observed in splicing factor 3b subunit 1 (SF3B1 R625H ), but its functional effects and underlying molecular mechanisms remain largely unexplored. Methods Using the CRISPR/Cas9 genome editing system and rat pituitary GH3 cells, we generated heterozygous Sf3b1 R625H mutant cells. Sanger and whole-genome sequencing were conducted to verify the introduction of this mutation. Transcriptome analysis was performed in SF3B1-wild-type versus mutant human prolactinoma samples and GH3 cells. RT-PCR and minigene reporter assays were conducted to verify aberrant splicing. The functional consequences of SF3B1 R625H were evaluated in vitro and in vivo. Critical makers of epithelial-mesenchymal transition and key components were detected using western blot, immunohistochemistry, and immunofluorescence. Suppressing proteins was achieved using siRNA. Results Transcriptomic analysis of prolactinomas and heterozygous mutant cells revealed that the SF3B1 R625H allele led to different alterations in splicing properties, affecting different genes in different species. SF3B1R625H promoted aberrant splicing and DLG1 suppression in both rat cells and human tumors. In addition, SF3B1R625H and knocking down DLG1 promoted cell migration, invasion, and epithelial-mesenchymal transition through PI3K/Akt pathway. Conclusions Our findings elucidate a mechanism through which mutant SF3B1 promotes tumor progression and may provide a potent molecular therapeutic target for prolactinomas with the SF3B1 R625H mutation.

【 授权许可】

Unknown