High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity

Journal of Lipid Research

Akheel A. Syed¹  Shaishav Dhage¹  Kwok Leung Ong¹  Fatiha Tabet¹  Handrean Soran²  Jan Hoong Ho³  Kerry-Anne Rye³  Safwaan Adam³  Basil J. Ammori³  Zohaib Iqbal³  Luisa F. Cuesta Torres⁴  Yifen Liu⁴ 

[1] Cardiovascular Trials Unit, Manchester University NHS Foundation Trust, Manchester, United Kingdom;Department of Surgery, Salford Royal NHS Foundation Trust, Salford, United Kingdom;Lipid Research Group, Division of Cardiovascular Sciences, University of Manchester, Manchester, United Kingdom;Lipid Research Group, School of Medical Sciences, University of New South Wales Sydney, Sydney, Australia;

关键词: micro-ribonucleic acid;    high-density lipoprotein functionality;    intercellular adhesion molecule-1;    weight loss;   

DOI  :  

来源: DOAJ

【 摘 要 】

Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders.

【 授权许可】

Unknown