Genetic assessment of three Fagaceae species in forest restoration trials

PeerJ

Patcharawadee Thongkumkoon¹  Sutthathorn Chairuangsri¹  Dia Panitnard Shannon¹  Pimubon Waikham¹  Waranee Pradit¹  Stephen Elliott¹  Jatupol Kampuansai¹  Prasit Wangpakapattanawong¹  Siriwadee Chomdej¹  Aizhong Liu² 

[1] Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand;Key Laboratory for Forest Resource Conservation and Utilization in the Southwest Mountains of China (Ministry of Education), Southwest Forestry University, Kunming, China;

关键词: Chiang Mai;    Framework tree species;    Forest restoration;    Genetic diversity;    Microsatellite markers;   

DOI  :  10.7717/peerj.6958

来源: DOAJ

【 摘 要 】

Restoring isolated patches of forest ecosystems in degraded landscapes could potentially lead to genetic loss and inbreeding. Therefore, this study determined the occurrence of genetic diversity among the tree species Castanopsis tribuloides, C. calathiformis, and Lithocarpus polystachyus all of which were proven previously to be effective native tree species in the restoration of upland evergreen forests in northern Thailand when using the seed sample collection method. We tested our hypothesis as to whether the genetic diversity of a plant population that had been planted from the seeds of 4–6 adult trees would be lower and whether incidences of fixation index (Fis) would be higher among the second generation seedlings of these three Fagaceae species in isolated forest restoration trial plots. Microsatellite primers were selected from the entire genome sequence of C. tribuloides and the genetic sequences of C. tribuloides, L. polystachyus, and C. calathiformis were analyzed. Our results indicated a high degree of genetic diversity (He) in C. tribuloides (0.736) and C. calathiformis (0.481); however, a low level of genetic diversity was observed in L. polystachyus (0.281) within the restored forest. The fixation index for the second generation of L. polystachyus and C. calathiformis in the restored forest showed evidence of inbreeding. These results imply the efficiency of the seed sample collection method and verify that it does not reduce the level of genetic diversity in C. tribuloides and C. calathiformis. However, it may result in incidences of an inbreeding phenomena, suggesting the need to increase the number of adult trees used at the seed collection stage.

【 授权许可】

Unknown