RNA-Seq Analysis of Cisplatin and The Monofunctional Platinum(II) Complex, Phenanthriplatin, in A549 Non-Small Cell Lung Cancer and IMR90 Lung Fibroblast Cell Lines

Cells

MichaelE. Smith¹  ElvinN. Irihamye¹  SatyaA. Moolani¹  JerryD. Monroe²  Yann Gibert²  JoshuaS. Speed³ 

[1] Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA;Department of Cell and Molecular Biology, University of Mississippi Medical Center, Jackson, MS 39216, USA;Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA;

关键词: next generation sequencing;    cancer;    monofunctional platinum(II) complex;    pathway analysis;    cisplatin;   

DOI  :  10.3390/cells9122637

来源: DOAJ

【 摘 要 】

Phenanthriplatin is a new monofunctional platinum(II) complex that binds only one strand of DNA and acts by blocking gene transcription, but its effect on gene regulation has not been characterized relative to the traditional platinum-based complex, cisplatin. A549 non-small cell lung cancer and IMR90 lung fibroblast cells were treated with cisplatin, phenanthriplatin, or a control and then their RNA transcripts were subjected to next generation sequencing analysis. DESeq2 and CuffDiff2 were used to identify up- and downregulated genes and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to identify pathways and functions. We found that phenanthriplatin may regulate the genes GPRC5a, TFF1, and TNFRSF10D, which act through p53 to control apoptosis, differently or to a greater extent than cisplatin, and that it, unlike cisplatin, could upregulate ATP5MD, a gene which signals through the Wnt/β catenin pathway. Furthermore, phenanthriplatin caused unique or enhanced effects compared to cisplatin on genes regulating the cytoskeleton, cell migration, and proliferation, e.g., AGAP1, DIAPH2, GDF15, and THSD1 (p < 0.05; q < 0.05). Phenanthriplatin may modulate some oncogenes differently than cisplatin potentially leading to improved clinical outcome, but this monofunctional complex should be carefully matched with cancer gene data to be successfully applied in chemotherapy.

【 授权许可】

Unknown