Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Catalysts

Mailin Misson¹  Sheng Dai²  Bo Jin³  Hu Zhang³ 

[1] Bioprocess Engineering Research Group, Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia;School of Chemical Engineering, Brunel University London, Kingston Lane, Uxbridge, Middlesex UB8 3PH, UK;School of Chemical Engineering, University of Adelaide, Adelaide, SA 5000, Australia;

关键词: β-galactosidase;    polymer nanofibers;    nanobiocatalyst;    interfacial characterization;    interfacial reaction;    conformational change;   

DOI  :  10.3390/catal10010081

来源: DOAJ

【 摘 要 】

Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

【 授权许可】

Unknown