First Report on Identification of Fusarium graminearum Species Complex Members from Turkey and Iran

Turkish Journal of Agriculture: Food Science and Technology

Bayram Kansu¹  Emre Yörük²  Özlem Sefer²  Berna Tunalı³  Bahram Sharifnabi⁴ 

[1] Animal and Plant Production Department, Vocational School, Samsun Ondokuz Mayis University, S55270 Atakum/Samsun;Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Yeni Yuzyil University, 34010 İstanbul;Department of Plant Pathology, Faculty of Agriculture, Samsun Ondokuz Mayis University, 55270 Atakum/Samsun;Department of Plant Protection, College of Agriculture, Isfahan University of Technology,;

关键词: dna sequencing;    fusarium graminearum;    fusarium head blight;    multilocus genotyping;    species complex;   

DOI  :  10.24925/turjaf.v7i7.1040-1045.2541

来源: DOAJ

【 摘 要 】

Fusarium graminearum species complex is the major Fusarium head blight disease pathogen in worldwide. Fusarium head blight disease lead to damages on small grain cereals. The identification of the F. graminearum species complex is important for improving disease control and management. Traditional identification methods such as macroscopy and microscopy analysis need to be supported by biochemical and genetics assays. Thus, DNA sequencing-based methods is one of the most preferred, reliable, low priced in the identification methods.In the study, 54 F. graminearum isolates obtained from diseased wheat, barley and corn fields in Turkey and Iran were identified by morphological characteristics and then characterized by species-specific SCAR marker. β-tubulin, Tef1-α, 28s rDNA and Histone H3 genes amplified, purified and then sequenced. The merged multiloci length was obtained as last of all 2215 bp. These sequencing results was used to multiloci genotyping assays. Last of all, 20 isolates were determined as F. graminearum sensu stricto by multiloci genotyping analysis. Remaining isolates were identified as F. asiaticum or Fusarium sp.. The findings are important in terms of revealing the first-time identification in Turkish and Iranian isolates as F. graminearum species complex members by amplification four (β-tubulin, Tef1-α, 28s rDNA and Histone H3) highly conserved DNA regions.

【 授权许可】

Unknown