A Chemical Investigation of the Leaves of Morus alba L.

Molecules

Xin Wang¹  MarkT. Hamann²  Xiao-yan Chen³  Jie Kang³  Ting Zhang⁴  Ruo-yun Chen⁵  De-quan Yu⁵ 

[1] Library, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100020, China;Beijing Key Laboratory of Bioactive Substances and Function Foods, Beijing Union University, Beijing 100191, China;Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA;;Institute of Medical Information &State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;

关键词: Morus alba L.;    aldose reductase inhibitor;    neuroprotective agent;    natural products;   

DOI  :  10.3390/molecules23051018

来源: DOAJ

【 摘 要 】

The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1–4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC50 values of 4.33 μM and 6.0 μM compared with the potent AR inhibitor epalrestat (IC50 1.88 × 10−3 μM). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001).

【 授权许可】

Unknown