Composite lymphoma of concurrent T zone lymphoma and large cell B cell lymphoma in a dog

BMC Veterinary Research

Nikos Darzentas¹  Arata Matsuyama²  Danielle Richardson³  Mei-Hua Hwang⁴  Dorothee Bienzle⁴  Nariman Deravi⁴  Stefan M. Keller⁴ 

[1] Department of Internal Medicine II, University Hospital Schleswig-Holstein, Kiel, Germany/Central European Institute of Technology, Masaryk University;Departments of Biomedical Sciences, University of Guelph;Departments of Clinical Studies, University of Guelph;Departments of Pathobiology, University of Guelph;

关键词: Canine;    Clonality;    Dog;    Lymphoma;    Lymphosarcoma;    Antigen receptor gene rearrangement;   

DOI  :  10.1186/s12917-019-2154-8

来源: DOAJ

【 摘 要 】

Abstract Background Evolution of indolent to aggressive lymphoma has been described in dogs but is difficult to distinguish from the de novo development of a second, clonally distinct lymphoma. Differentiation of these scenarios can be aided by next generation sequencing (NGS)-based assessment of clonality of lymphocyte antigen receptor genes. Case presentation An 8-year-old male intact Mastiff presented with generalized lymphadenomegaly was diagnosed with nodal T zone lymphoma (TZL) based on cytology, histopathology, immunohistochemistry and flow cytometry. Thirteen months later, the dog re-presented with progressive lymphadenomegaly, and based on cytology and flow cytometry, a large B cell lymphoma (LBCL) was diagnosed. Sequencing-based clonality testing confirmed the de novo development of a LBCL and the persistence of a TZL. Conclusions The occurrence of two distinct lymphoid neoplasms should be considered if patient features and tumor cytomorphology or immunophenotype differ among sequential samples. Sequencing-based clonality testing may provide conclusive evidence of two concurrent and distinct clonal lymphocyte populations, termed most appropriately “composite lymphoma”.

【 授权许可】

Unknown