Transcriptomic Signatures and Upstream Regulation in Human Skeletal Muscle Adapted to Disuse and Aerobic Exercise

International Journal of Molecular Sciences

DaniilV. Popov¹  PavelA. Makhnovskii¹  RomanO. Bokov¹  FedorA. Kolpakov² 

[1] Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia;Institute of Computational Technologies of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia;

关键词: transcriptome;    disuse;    transcription factors;    heterodimer;    extracellular matrix;    mitochondrion;   

DOI  :  10.3390/ijms22031208

来源: DOAJ

【 摘 要 】

Inactivity is associated with the development of numerous disorders. Regular aerobic exercise is broadly used as a key intervention to prevent and treat these pathological conditions. In our meta-analysis we aimed to identify and compare i) the transcriptomic signatures related to disuse, regular and acute aerobic exercise in human skeletal muscle and ii) the biological effects and transcription factors associated with these transcriptomic changes. A standardized workflow with robust cut-off criteria was used to analyze 27 transcriptomic datasets for the vastus lateralis muscle of healthy humans subjected to disuse, regular and acute aerobic exercise. We evaluated the role of transcriptional regulation in the phenotypic changes described in the literature. The responses to chronic interventions (disuse and regular training) partially correspond to the phenotypic effects. Acute exercise induces changes that are mainly related to the regulation of gene expression, including a strong enrichment of several transcription factors (most of which are related to the ATF/CREB/AP-1 superfamily) and a massive increase in the expression levels of genes encoding transcription factors and co-activators. Overall, the adaptation strategies of skeletal muscle to decreased and increased levels of physical activity differ in direction and demonstrate qualitative differences that are closely associated with the activation of different sets of transcription factors.

【 授权许可】

Unknown