The Expression Profile of OSBP, CAT and BZIP Genes in Drought Tolerant and Susceptible Soybean Cultivars Using Real Time PCR

مجله بیوتکنولوژی کشاورزی

关键词: : qrt-pcr;    stress;    osbp;    cat;    bzip;    soybean;   

DOI  :  10.22103/jab.2014.1323

来源: DOAJ

【 摘 要 】

Under drought stress, a signaling system induces expression of specific genes to alleviate the harmful effects of drought stress. BZIP gene is a transcription factor in the signaling of abiotic stress and plays a role in the regulation responses to different stresses in plants and activated by ABA and closing the stomata. OSBP gene plays a key role in the signaling in several physiological reactions in response to stress. Catalase enzyme is series of anti-oxidant, which catalyzes the conversion of hydrogen peroxide to water and oxygen molecules. Two soybean cultivars, Williams (tolerant to drought) and L17 (susceptible to drought) were cultured in the greenhouse conditions. Drought stress treatment was performed at two-leaf stage for 7 days. Total RNA was extracted from leaves and roots of both control and stressed plants. Then cDNA was synthesized and used for Real time PCR. To normalize data the housekeeping gene 18SrRNA was used. Data analysis based on Ct curves showed that the expression of BZIP gene increased under drought stress in both leaf and root. The BZIP gene expression in Williams was two-fold greater than L17 cultivar. Also its expression was higher in root than leaf. OSBP gene expression differences were not significant between Williams and L17. The expression of CAT gene in Williams was two-fold greater than L17, and its expression was higher in root than leaf. According to increasing of drought tolerance through the expression of these genes, it can be concluded that transferring of these genes may enhance drought tolerance in high yield soybean genotypes

【 授权许可】

Unknown