Inhibition of Acetylcholinesterase Modulates NMDA Receptor Antagonist Mediated Alterations in the Developing Brain

International Journal of Molecular Sciences

Marco Sifringer¹  Clarissa von Haefen¹  Fatme Nasser¹  Claudia D. Spies¹  Benjamin Rohrer¹  Stefanie Endesfelder²  Josephine Herz³  Meray Serdar³  Ursula Felderhoff-Mueser³  Ivo Bendix³ 

[1] Department of Anesthesiology and Intensive Care Medicine, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum, Berlin 13353, Germany;Department of Neonatology, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum, Berlin 13353, Germany;Department of Pediatrics I, Neonatology, University Hospital Essen, Essen 45122, Germany;

关键词: developing brain;    NMDA receptor;    MK801;    acetylcholinesterase;    extracellular matrix;    BDNF;    neuroprotection;   

DOI  :  10.3390/ijms15033784

来源: DOAJ

【 摘 要 】

Exposure to N-methyl-d-aspartate (NMDA) receptor antagonists has been demonstrated to induce neurodegeneration in newborn rats. However, in clinical practice the use of NMDA receptor antagonists as anesthetics and sedatives cannot always be avoided. The present study investigated the effect of the indirect cholinergic agonist physostigmine on neurotrophin expression and the extracellular matrix during NMDA receptor antagonist induced injury to the immature rat brain. The aim was to investigate matrix metalloproteinase (MMP)-2 activity, as well as expression of tissue inhibitorof metalloproteinase (TIMP)-2 and brain-derived neurotrophic factor (BDNF) afterco-administration of the non-competitive NMDA receptor antagonist MK801 (dizocilpine) and the acetylcholinesterase (AChE) inhibitor physostigmine. The AChE inhibitor physostigmine ameliorated the MK801-induced reduction of BDNF mRNA and protein levels, reduced MK801-triggered MMP-2 activity and prevented decreased TIMP-2 mRNA expression. Our results indicate that AChE inhibition may prevent newborn rats from MK801-mediated brain damage by enhancing neurotrophin-associated signaling pathways and by modulating the extracellular matrix.

【 授权许可】

Unknown